What makes a good mammalian 3'UTR?
ladasky at leland.Stanford.EDU
Mon Mar 24 16:59:16 EST 1997
I've been working with a non-commercial eukaryotic expression vector
and I have been having trouble getting readable sequence. I designed three
different primers that sit down in the region immediately 3' of the poly-
linker, and they all gave strong background bands. After much haranguing, I
obtained an official sequence for this plasmid. Surprise! The 3' UTR that
is present next to the polylinker is also present downstream of the eukary-
otic neomycin resistance gene. The duplicated region is nearly 300 base
I am considering altering this plasmid so that the sequence next to
the polylinker appears only once, as it should. I am wondering how hard it
is to change the 3' UTR for the neoR gene. Is simply placing the AATAAA
sequence a few hundred bp downstream of the gene adequate? Or are there
other helpful signals? Should I just grab a 3' UTR from another gene?
Unique ID : Ladasky, John Joseph Jr.
Title : BA Biochemistry, U.C. Berkeley, 1989 (Ph.D. perhaps 1998???)
Location : Stanford University, Dept. of Structural Biology
Keywords : immunology, music, running, Green
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