Q. electroporation protocols wanted

[Michael Hodges]

Dear Newswatchers

Wanted: electroporation protocol details for transformation of plant
protoplasts (especially tobacco mesophyll cell protos) so as to carry out
transient expression studies......please state electroporation buffer,
plasmid concentration, field strength, capacitance, pulse time constant
and any other important overlooked details. Please send your help directly
to my e-mail address: hodges at sidonie.ibp.u-psud.fr

Thanking you all in advance