Urea-containing buffers: How long can I store them?

[Achim Recktenwald, PhD]

packard at kersur.net wrote:
> 
> >Viraj Master wrote:
> >>
> >> Hello all,
> >>
> >> I am using the Qiagen pQE series of vectors to express proteins.  There
> >> are two buffers which I use a lot, Buffer B and Buffer C.
> >>
> >> These contain:
> >> 8M urea
> >> 0.1M NaH2PO4
> >> 0.01M Tris
> >>
> >> Buffer B is at pH=8
> >> Buffer C is at pH=6.3
> >>
> >> I have been told that I must make these buffers up **fresh** each time
> >> prior to use.  I would like to be able to prepare batches of these
> >> buffers and store them.  Does anybody know whether this can be done and
> >> not affect the protein prep?  If so, are they stored in the cold, or at
> >> room temp?  For how long can they be stored?
> >>
> >> Many thanks,
> >>
> >> Viraj
> >>
> >> Viraj Master PhD
> >> Dept of Organismal Biology
> >> University of Chicago
> >
> >
> 
>         I work for a biotech company, and we use 8M urea buffers on
> occasion.  We assign a 3 day expiration on these buffers.  I hope this
> helps.
> 
>                                         Kevin M Packard



The pH 8 buffer I would prepare fresh every day. For the pH 6.3 buffer
the 3 days expiration advice sounds good.

Achim

_____________
Achim Recktenwald, PhD.
IBEX Technologies, Inc.
Biochemistry
5485 rue Pare
Montreal, Quebec, H4P 1P7
Canada
achim at ibex.ca


The usual disclaimers apply.