Help gels cracking

Achim Recktenwald, PhD achim at
Mon Mar 24 08:44:41 EST 1997

Gina Berardesco wrote:
> > Although her 8% acrylamide sds gels dry down fine (normaly
> > within 2-3hrs) her 10% gels are cracking on the gel dryer.
> > She is pre-wetting her whatman and drying down at 80 degrees
> > centigrade...
> I've heard from a labmate that using old TEMED can cause this to happen,
> too. (Although I supposed both types of gel would be cracking if this were
> the problem.)

The acrylamide solution might be too old. Acrylamide decomposes to
acrylic acid during storage. 


Achim Recktenwald, PhD.
IBEX Technologies, Inc.
5485 rue Pare
Montreal, Quebec, H4P 1P7
achim at

The usual disclaimers apply.

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