Help gels cracking

[Achim Recktenwald, PhD]

Gina Berardesco wrote:
> 
> > Although her 8% acrylamide sds gels dry down fine (normaly
> > within 2-3hrs) her 10% gels are cracking on the gel dryer.
> > She is pre-wetting her whatman and drying down at 80 degrees
> > centigrade...
> 
> I've heard from a labmate that using old TEMED can cause this to happen,
> too. (Although I supposed both types of gel would be cracking if this were
> the problem.)
> 


The acrylamide solution might be too old. Acrylamide decomposes to
acrylic acid during storage. 

Achim 

_______________________
Achim Recktenwald, PhD.
IBEX Technologies, Inc.
Biochemistry
5485 rue Pare
Montreal, Quebec, H4P 1P7
Canada
achim at ibex.ca


The usual disclaimers apply.