Bacterial DNA for PFGE

[Yves Bertheau]

Hello,

We started a comparison of the PFGE patterns of pectinolytic erwinias
(enterabacteria).

We have troubles with some strains whose DNA get a smear in all
tested  conditions (even with 1 min at 100  C before lysis).

Does anybody know a protocol to avoid this DNA's degardation.

Thanks in adavance for your help

		Yves Bertheau

PS/ could you send a copy of your message to my e-mail?
Thanks