Direct or Nested PCR?

ma ddsyr at PUBLIC.EAST.CN.NET
Mon Mar 24 06:56:01 EST 1997


Dear Neters
I want cloning a gene promoter from Arabidopsis. I know the sequence. And this gene is 
the single copy. the promoter region is about 1100bp long. I am not sure whether I 
should direct PCR this promoter(using one pair of primers) or Nested PCR (using two 
pairs of primers). Anybody has the experiences on this aspect is welcome.
Ma



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