dissolving of RNA

Susan Hogarth sjhogart at unity.ncsu.edu
Mon Mar 24 23:18:02 EST 1997


David F. Spencer wrote:


<snip> 
> As to this (apparently popular) notion that you can overdry an RNA pellet, let
> me state that this is pure hogwash.  If RNA is properly desalted then you can
> dry it for a week and it will redissolve just fine.  Note that this is quite
> different from the situation for larger quantities of even well washed high
> molecular weight (greater than 20-30 kb) _DNA_.  In this case it is definitely
> unwise to do more than a light drying if you wish to get the DNA
> resolubilized in less than days or weeks.

Again; why? Is it the residual salt?
-- 
Susan
http://www4.ncsu.edu/unity/users/s/sjhogart/public/home.html

"To stay awake all night adds a day to your life."
                       -- Stilgar (Frank Herbert)



More information about the Methods mailing list