Yeast two hybrid

Steve Cohen sbc4 at PO.CWRU.EDU
Tue Mar 25 08:11:16 EST 1997


I need some input...

I am attempting to see if my bait, a Lex fusion, is being expressed in
yeast before transfecting my library. The yeast strain is TAT7, a L40
sub-strain.

I am attempting to preform a Western on yeast extracts which I have made
using zymase and sonication. My primary Ab is a Lex polyclonal from SANTA
CRUZE. Secondary from them too.

Has anyone used thief antibodies to Lex? Are their other Ab available for
Lex? 
Any other advise on how to maximize expression from yeast, and detect
expressed proteins would be helpful.

Thanks

Steve




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