DNA fingerprinting mismatch
ladasky at leland.Stanford.EDU
Tue Mar 25 05:31:09 EST 1997
In article <33376911.4EB2 at false.com>,
Ronnie J. Dean <ronjdean at false.com> wrote:
>Not sure your methodology but keep in mind that gametes are haploid.
>As for differences in other cells, the distribution of "active" and Barr
>Body chromosomes in females is more or less random ex. calico cats.
Unless you have to do single-cell PCR before you do your finger-
printing, then a gamete sample, consisting of many cells, should have the
same fingerprint as the diploid. As for the question of Barr bodies
(inactivated X chromosomes), what credible fingerprinting technique would
not extract the nucleoproteins while preparing the DNA? Again, the finger-
print of the adult somatic cell sample should be the same as a germ line
cell prior to dosage compensation.
Unique ID : Ladasky, John Joseph Jr.
Title : BA Biochemistry, U.C. Berkeley, 1989 (Ph.D. perhaps 1998???)
Location : Stanford University, Dept. of Structural Biology
Keywords : immunology, music, running, Green
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