DNA fingerprinting mismatch

John Ladasky ladasky at leland.Stanford.EDU
Tue Mar 25 05:31:09 EST 1997


In article <33376911.4EB2 at false.com>,
Ronnie J. Dean <ronjdean at false.com> wrote:
>Not sure your methodology but keep in mind that gametes are haploid. 
>As for differences in other cells, the distribution of "active" and Barr
>Body chromosomes in females is more or less random ex. calico cats.


	Unless you have to do single-cell PCR before you do your finger-
printing, then a gamete sample, consisting of many cells, should have the 
same fingerprint as the diploid.  As for the question of Barr bodies
(inactivated X chromosomes), what credible fingerprinting technique would
not extract the nucleoproteins while preparing the DNA?  Again, the finger-
print of the adult somatic cell sample should be the same as a germ line
cell prior to dosage compensation.


-- 
Unique ID : Ladasky, John Joseph Jr.
Title     : BA Biochemistry, U.C. Berkeley, 1989  (Ph.D. perhaps 1998???)
Location  : Stanford University, Dept. of Structural Biology
Keywords  : immunology, music, running, Green



More information about the Methods mailing list