Yeast nucleus staining

Michael Lichten lichten at helix.nih.gov
Tue Mar 25 09:18:47 EST 1997


In article <33368E33.41C6 at po.uni-stuttgart.de>, Martin Ligr
<martin.ligr at po.uni-stuttgart.de> wrote:

> Hello:
> I am currently using DAPI to visualize yeast nuclei, but the signal from
> nucleus is often obscured by strong signal from mitochondria. So I am
> thinking about using Hoechst 33258 or 33342. Could someone give me some
> info what is the difference between these two and how do they compare to
> DAPI? And is there any nucleus specific stain at all?
> Thanks,
> Martin Ligr

I believe that all, unfortunately, preferentially stain AT-rich sequences,
which is why the high mitochondrial staining.  You may wish to try
propidium iodide, which in our hands gave much less mitochondrial
background.

-- 
Michael Lichten
lichten at helix.nih.gov



More information about the Methods mailing list