rollins.24 at postbox.acs.ohio-state.edu
Tue Mar 25 19:26:58 EST 1997
I've read several times that it is preferable to linearize my vector for T7 in
vitro transcription with a UNIQUE restriction site. Does this mean the entire
plasmid should have only one of the required sites (to terminate the
transcript)? Or does this mean to make sure that your desired transcript
sequence does not contain this site? If the entire plasmid should have only
one of the linearization sites, why is this necessary? Do extraneous
linear DNA fragments somehow inhibit T7 pol from recognizing the T7 promoter?
Any advice would be greatly appreciated.
Rollins.24 at osu.edu
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