help for reducing sugar analysis
D. Vadnais
dvadnais at uoguelph.ca
Wed Mar 26 13:36:45 EST 1997
I have tried to use the Nelson-Somogyi method for determining reducing
sugars released by invertase activity. I have not been able to make it
work because of bubbles forming on the sides of the cuvette when I put
the samples into the spectrophotometer. I was wondering if anybody else
has had this problem, and what they did to correct it. I have tried the
method as outlined by Somogyi in 1951. Any suggestions? Please reply to
my e-mail address: dvadnais at crop.uoguelph.ca
Thanks.
Dave
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