cDNA protocols

Robert Pomponio, Ph.D. rpomponio at genzyme.com
Wed Mar 26 10:50:41 EST 1997


In response to your question about cDNA kits... I've tried and beta-tested
quite a few of them out there and the most reliable one I've used is the
Pharmacia First-strand cDNA synthesis kit.  I've done a head to head
comparison of their Ready-to-go cDNA kit, and the results were not as
consistent as the regular old kit.

Hope this helps
Rob Pomponio
Genzyme Genetics
Framingham, MA




In article <01bc34b8$ae9728a0$d966c180 at geneticslab2>, "John Korte"
<kortej at css.orst.edu> wrote:

> Greetings,
> I am preparing total RNA via Tri reagent, and was wondering about the
> current popularity of cDNA kits or protocols.  If you have a protocol that
> works well I would appreciate a copy of it.
> 
> Thanks,
> John Korte
> kortej at css.orst.edu
> Oregon State Univ.
> Crop & Soil Science
> Corvallis, OR  97331
> fax (541)737-1589



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