How to get rid of extra A added by Taq

Dimitri Harmegnies ldewit at ben.vub.ac.be
Wed Mar 26 03:50:30 EST 1997


You can probably blunt your first PCR product by T4 DNA polymŽrase wich
have a 3'-5' exonuclease activity.

Dimitri Harmegnies
ldewit.ben.vub.ac.be
Dept. of Virology
Institut Pasteur de Bruxelles
642 rue Engeland
B-1180
Brussels, Belgium



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