autofluorescence

Bill Kalionis ogbk at gamgee.cc.flinders.edu.au
Wed Mar 19 17:38:23 EST 1997


We are having problems with cells that autofluoresce brightly in the rhodamine 
channel and to a lesser degree in the fluorescein channel. Does anybody have 
any suggestions as to how to quench autofluorescence. I have heard that 
crystal violet and trypan blue are supposed to do this. Does anyone have 
experience with these reagents for this purpose?

Thanks for your help



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