In vitro transcription/translation

Will Fuller wf10000 at cus.cam.ac.uk
Thu Mar 27 12:33:00 EST 1997


Hi
This may be very obvious but I didn't spot it. It is essential that you have a kkozak sequence in your expression plasmid otherwise no matter which polymerase you use you'll see no protein with the Promega kits

Good luck

Will



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