dissolving of RNA
Paul A Bucciaglia
bucci001 at maroon.tc.umn.edu
Fri Mar 28 14:43:43 EST 1997
I missed the original post and did not catch which organism the RNA is
being extracted from, but at least with plants another alternative exists
besides over-drying the pellet--the RNA is contaminated with phenolics and
carbohydrates. And not just contaminated; often they are covalently
linked to the nucleic acids. There are publisshed protocols which can
overcome this; if anyone is interested I'll post them.
jerikse at bsd.meddean.luc.edu (Jason Eriksen) writes:
>xudong huang <xudong.huang at endo.mas.lu.se> wrote:
>>It is the problem of over-drying. To avoid it, just pipette away
>>remaining ETOH drops as much as you can, air-dry the pellet 5-10 min at
>>room temperature until you can not see ETOH drops, then add DEPC water.
>>It should be dissolved. Hope it helps.
>This is excellent advice! I ran into the same problem when I was first
>learning RNA extractions; what most beginners do, unless they know
>better, is to really try to get that pellet dry. Of course, what
>happens is that you get a firm, translucent, non-dissolving pellet of
>It took me a month of effort to figure out that this was the problem.
>Xudong is right on the money. Don't dry your pellet too much, and do
>avoid subjecting it to 65 C temperatures. Room temperature for drying
>is just fine.
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