Boiling RNA

Jim WIck alpha01 at execpc.com
Fri Mar 28 08:37:35 EST 1997


To extract nucleic acid from cryptosporidium I have to do repeatitive 
freezing/thawing followed by 30 min boiling of my sample. I am 
wondering if the boiling is trashing my RNA since I would like to 
RT-PCR a mRNA aside from PCR from the isolated DNA?




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