Yeast nucleus staining

Chong Jue jue at genectr.hunter.cuny.edu
Mon Mar 31 21:35:10 EST 1997


Michael Lichten wrote:
> 
> In article <33368E33.41C6 at po.uni-stuttgart.de>, Martin Ligr
> <martin.ligr at po.uni-stuttgart.de> wrote:
> 
> > Hello:
> > I am currently using DAPI to visualize yeast nuclei, but the signal from
> > nucleus is often obscured by strong signal from mitochondria. So I am
> > thinking about using Hoechst 33258 or 33342. Could someone give me some
> > info what is the difference between these two and how do they compare to
> > DAPI? And is there any nucleus specific stain at all?
> > Thanks,
> > Martin Ligr
> 
> I believe that all, unfortunately, preferentially stain AT-rich sequences,
> which is why the high mitochondrial staining.  You may wish to try
> propidium iodide, which in our hands gave much less mitochondrial
> background.
> 
> --
> Michael Lichten
> lichten at helix.nih.gov
Michael,

I did try to use propidium iodide to stain yeast nuclei.  I could only
see dead cells picking the dye and live cells simply excluded the dye. 
Do you (or anyone) know of an easy way to permeablize the cells for PI
staining?
Regards.

Chong

----
Chong K. Jue
Dept of Biological Sciences
Hunter College
New York, NY 10021
USA
Email: jue at genectr.hunter.cuny.edu
----



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