Northern with 40mer oligo
marion.patrice at uqam.ca
Mon Mar 31 13:15:54 EST 1997
Excuse my english, I'm french speaking.
Is anyone have a GOOD protocole for Northern hybridization with a 40mer
oligo ? I have look at several formulas to figure out the Tm and all of
them give different results. I am working with hybridization solution
containing 5 X SSPE, no formamide, my oligos are 52% to 54% GC (100%
homology). The Tm-25 I obtained (for hybridization) is around 37 C. Does
it look right ?
What kind of wash solution sould I use ? Can I go high stringency with
oligos (i.e. 0,2 SSC) without loosing the signal?
Thanks in advance,
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