Minéral Oil - PCR
Dr. Duncan Clark
duncan at genesys.demon.co.uk
Mon Mar 31 12:42:52 EST 1997
In article <5he8i5$1213 at ncisun1-nf0.ncifcrf.gov>, Paul N Hengen
<pnh at ncifcrf.gov> writes
>Dr. Duncan Clark (duncan at genesys.demon.co.uk) wrote:
>> I leave this oil since one
>> month under a laminar hood, with UV exposition every night.
>| Mineral oil can go off and the UV is the likely source. I think this or
>| something similar has been reported either in Biotechniques or NAR.
>Can you please post this reference?
I wish I knew what it was but I have definitely seen something about
mineral oil going off. We still have a couple of PCR machines without a
hot lid and the mineral oil we use is Sigma Cat. No. M5904. The main
stock bottle is at least a couple of years old but is still OK and we
actually autoclave 30ml at a time when required.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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