Methods March 1997 Archives by author

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Starting: Sat Mar 1 04:45:31 EST 1997
Ending: Mon Mar 31 21:35:10 EST 1997
Messages: 850

• detection of proteins in FPLC   "ITABAJARA_DA_SILVA_VAZ_JU,ita at DNA.CBIOT.UFRGS.BR" at invivo.edu
• Hydroxylamine cleaveage at N/G residues of protein   "Marianne Leverone ", BIO
• plamid screening   "Srikumar_M._Raja,raja at uvic.ca" at invivo.edu
• ampicillin analog?   "mstanbro at bih.harvard.edu" at invivo.edu
• problems with GST-peptide binding after Sarkosyl lysis  
• How to separate vegf dimer into monomer?   11
• RNA storage in formamide   DIEGO BONATTO - 219
• Best protocol for RNA extraction of S. cerevisiae   DIEGO BONATTO - 219
• Pvu II partial digest conditions needed   :-Peter
• Tris-Tricine protocols   Volker Nölle
• Immunoglobulin chimera between Heavy chain and protein   at po.cwru.edu
• Cs-gluconat wanted!!   at uni-tuebingen.de
• Test from BIONET staff: ignore   A.Bleasby
• oligo biotinylation?   AAAACc
• desperately need help   AAAACc
• desperately need help   AAAACc
• SP6 RNA Polymerase Gene   Dr. Stuart A. ALI
• G418 and SF9 cells: Help   LUIS BEJARANO ARDURA
• monocyte cell line for IP   Yasuhito Abe
• Ion Exchange Chro/HPLC with 6M Guanidine or Some Detergent?   Achim Recktenwald, PhD
• detection of proteins in FPLC   Achim Recktenwald, PhD
• Dye-binding protein assay giving very low signals - any ideas?   Achim Recktenwald, PhD
• Help gels cracking   Achim Recktenwald, PhD
• Urea-containing buffers: How long can I store them?   Achim Recktenwald, PhD
• I have money to spend...   Ken Adams
• BIOSCI/bionet miniFAQ & Fundraiser   BIOSCI Administrator
• Primers annealing protocol   Ashok Aiyar
• How to re-amplify the DDRT-PCR band?   Louis Alarcon
• western blot   M Allen
• B-M Titan RT-PCR   M Allen
• RT-PCR HEEELP!   Karen Allendoerfer
• Looking for Gel analysis software for Mac   Paul St. Amand
• Scanners reply   Javier Ambrosio
• Epitope Tagging Questions...   Thomas R. Anderson
• Readytogo dried(?) ligase   Andy
• 293 cells with transformed phenotype?   Andy
• (no subject)   Mark Arboleda
• pET   Shiwani Arora
• Tris-Tricine protocols   Shiwani Arora
• In vitro transcription with T7 RNAP   ISOAI Atsushi
• automated sequencing   ISOAI Atsushi
• VEGF dimers and multimers   Cara Baker
• Detergents function??   Balwi
• Paraformaldehyde and autofluorescence.   Balwi
• PCR product in wells   Wayne M. Barnes
• Coverslip-base culture vessels   Derek Bartlem
• Plant tissue PCR   Brett Beitzel
• Whole genome amplification?   Brett Beitzel
• matrix for spin columns   Michael Benedik
• MacConkey instead of XGAL ?   Michael Benedik
• G-banding mouse spreads   W R Bennett
• FISH probe: random prime or nick translate?   W R Bennett
• FISH probe random prime or nick translate   W R Bennett
• detection of proteins in FPLC   W R Bennett
• cross-linker   W R Bennett
• detection of proteins in FPLC   W R Bennett
• looking for multiple-Nucleic Acid sequences alignment software for PCs   W R Bennett
• Help gels cracking   Gina Berardesco
• Ribonuclease protection assay - fluorescent ribonucleotides   David T. Berg
• dissolving of RNA   David T. Berg
• sequensing no problem   Hallgeir Bergum
• Malachite green   Bergwerff
• Seeking gyrA462 CcdR E coli mutant   Mary Berlyn
• Bacterial DNA for PFGE   Yves Bertheau
• At 50% Commission....   Bio at GINETTE.COM
• (none)   Shaun D. Black
• Reference for mouse and human codon frequency   Shaun D. Black
• (none)   Shaun D. Black
• Freeze & squeeze w/ PAGE?   Volker Blaschke
• quikchange question   Thorarinn Blondal
• ABI DNA sequencer--wanted   Kevin S. Bodner
• Invitrogen vectors   F. Boeckman
• detection of proteins in FPLC   Mario J. Borgnia
• How to solublize bacterial membrane   Mario J. Borgnia
• How to get rid of extra A added by Taq   Joe Boutell
• Urea-containing buffers: How long can I store them?   Joe Boutell
• Looking for Gold Biotechnology   Joan Boyce
• Best way to search the 'net for reagents   Joan Boyce
• E. coli genotypes   Chris Boyd
• Q: solublility of CsCl in EtOH and iPropOH? (recovery of plasmid   Chris Boyd
• phosphatase question (library construction)   Alex Brands
• In vitro transcription/translation   Bresagen
• Sizes of 28S, 18S & 7S rRNA   Pak Brian
• Error bar in Microsoft Excel 5.0?   Helgi Briem
• best library kit?   Deb Britt
• IMPORTANT ** Cytokines Web Announcement   Simon M. Brocklehurst
• His-tag protein purification   Bud Brown
• In vitro translation of pre-mRNA   Steve Brown
• native e. coli lysis/ inexpensive sonicator supplier source?   Steve Brown
• Generunner/Hastings Software   Karen S. Browning
• dissolving of RNA   Paul A Bucciaglia
• recombinant plasmid screening   Tom Buckley
• Dye-binding protein assay giving very low signals - any ideas?   Dr E. Buxbaum
• pET   Yvon CAVALOC
• gel shift assay   Yvon CAVALOC
• ampicillin analog?   Yvon CAVALOC
• Whole genome amplification?   Yvon CAVALOC
• Sedimentation of Splicing stuff!   Yvon CAVALOC
• nested-PCR?   Yvon CAVALOC
• How to clone a 20 bp fragment?   Yvon CAVALOC
• PCR cloning   Yvon CAVALOC
• Primers annealing protocol   Yvon CAVALOC
• Pipettor opinions   Francis Chun-wai CHAN
• Differential Display of cartilage cell differentiation   Francis Chun-wai CHAN
• Gene bank of mice   Francis Chun-wai CHAN
• ampicillin analog?   MR ROBERT G CHAPMAN
• lactamase inhibitor?   MR ROBERT G CHAPMAN
• p53 Assay   Lou Cantolupo
• rDNA Techniques & Applications Workshop   Mr. Ken Carter
• PCR Techniques & Applications Workshop   Mr. Ken Carter
• rDNA Workshop Final Announcement   Mr. Ken Carter
• PCR Workshop Final Announcement   Mr. Ken Carter
• NEN renaissance kit   Ron Caspi
• IMMUNOFLUORESCENCE COURSE   Paolo Castano
• Wanted: 18S or 28S probe   Edmundo Castro
• Where to buy NP-40? (Not Sigma)   Alberto Catalano
• FISH probe: random prime or nick translate?   Alberto Catalano
• What is Decon 90?   Mike Challen
• How to solublize bacterial membrane   Alex Chang
• detection of proteins in FPLC   Tom Chappell
• DNA Auto-sequencing   Tom Chappell
• Name and Gene map for plasmid-CoxII   Geoffrey Charters
• Problems with biolistics protocol   Don Chen
• Centricon concentrator re-use   Frances E. Chen
• oligo biotinylation?   Casey Chin
• plasmid copy number estimation in E.coli   Choi, Seung Jin
• rRNA contamination of mRNA preps   Merrill Christensen
• distinct bands in the slot of gel   Shi-Jye Chu
• distinct band in begining of the slot in gel   Shi-Jye Chu
• Error bar in Microsoft Excel 5.0?   Namjin Chung
• 4% Agar?   Namjin Chung
• Toothpicks?   Namjin Chung
• Yeast Strain Cataloging Software!   Namjin Chung
• Help: looking for Arthrobacter DNA extraction protocol   Maurizio Cirilli
• Help! PCR linearity   Dr. Duncan Clark
• Tli polymerase for PCR...   Dr. Duncan Clark
• Uracil containing DNA and Proof-reading pols   Dr. Duncan Clark
• T7 select phage display   Dr. Duncan Clark
• SP6 RNA Polymerase Gene   Dr. Duncan Clark
• Readytogo dried(?) ligase   Dr. Duncan Clark
• ampicillin analog?   Dr. Duncan Clark
• BSA in PCR reaction: role of?   Dr. Duncan Clark
• Pvu II partial digest conditions needed   Dr. Duncan Clark
• Minéral Oil - PCR   Dr. Duncan Clark
• Minéral Oil - PCR   Dr. Duncan Clark
• Semi-Dry Transfer Apparatus Reccomendations   William Coetzee
• pHOOK plasmid- Capture of transient transfected cells   Steve Cohen
• Yeast two hybrid   Steve Cohen
• Heparin coated microfuge tubes   Dr.W.H. Colledge
• Sectioning mouse eyes Help!   Allan Colthart
• Wanted: 18S or 28S probe   Allan Colthart
• extracting blue dextran and leaving behind DNA?   Richard Converse
• tempest on a toothpick   Tom Cooper
• pre-made Northern blots   Francois Coulier
• preferential amplification by proofreading Taq ???   DHolst6016
• Semi-Dry Transfer Apparatus Reccomendations   DSF
• western blot   DSF
• Error bar in Microsoft Excel 5.0?   Douglas Danforth
• Error bar in Microsoft Excel 5.0?   Douglas Danforth
• Dye-binding protein assay giving very low signals - any ideas?   David
• Storing RNA in formamide: The Downside?   David L. Haviland, Ph.D.
• IgG conc in ascites?   David L. Haviland, Ph.D.
• RNA storage in formamide   David L. Haviland, Ph.D.
• lactamase inhibitor?   David L. Haviland, Ph.D.
• lactamase inhibitor?   David L. Haviland, Ph.D.
• DNA fingerprinting mismatch   Ronnie J. Dean
• BSA in PCR reaction: role of?   Deborah.Hailstones at SMTPGWY.AGRIC.NSW.GOV.AU
• Two-hybrid question   Mohanish Deshmukh
• used lab equipment   Therese Ann Dietlin
• Membrane protein prep   Ole Diettrich
• Sterilisation of Bead-coupled antibodies   Ole Diettrich
• DNA with RNA   Alex Dobrovic
• deionised formamide   Alex Dobrovic
• :please maximise information in your email subjects   Alex Dobrovic
• impurities in SDS (Lauryl sulfate, sodium salt)   Alex Dobrovic
• RT-PCR on formalin fixed paraffin sections   Alex Dobrovic
• multiple sequence alignment software for MACs   Alex Dobrovic
• Blunt-end troubles   Andrew Doherty
• cpm vs. dpm ?   Andrew Doherty
• flounder (or similar) cDNA expression library please   Colin Dolphin
• Announcing "Q-RT-PCR": Software for quantitative RT-PCR   Peter Doris
• Plasmid pSPTBM20/21   Scott Dorland
• Antisense transcripts   Julian Dow
• Question about genomic library construction   Xiaohan Du
• SSB   Xiaohan Du
• Cheapest "clean" Taq polymerase?   Richard J. Dudley
• dissolving of RNA   Richard J. Dudley
• HELP! Triton X114 blues...   Steve Dunn
• Kidney glomeruli mRNA prep. ?   Dan Eilat
• Storing RNA in formamide: The Downside?   Jason Eriksen
• Cheaper Trizol Alternative?   Jason Eriksen
• dissolving of RNA   Jason Eriksen
• E.M. Imaging   Orhan Ertughrul
• Question about pJM17   Klaus Eschrich
• Fellow Biochemists : Looking for advice   Hernan Espinoza
• cpm vs. dpm ?   Judith Ann Eudaly
• Software for RNA 2-D structure analysis   Tatsuhiro Ezawa
• RNA probe loop formation analysis   Tatsuhiro Ezawa
• Min éral Oil - PCR   Labo Tim ETS FC
• [Q] MAPK inhibitors   FX
• Tris-Tricine protocols   Dr. Frank O. Fackelmayer
• ADN/derivatization/TMS/bases   Fadi.Adas
• DNA/Guanine/TMS derivatization???   Fadi.Adas
• UV crosslinking   Levon Fendekian
• cross-linker   Levon Fendekian
• determining titer   Karl Fischer
• FISH probe random prime or nick translate   Karl Fischer
• Urea-containing buffers: How long can I store them?   David B. Flora
• What is a "trypsinizing flask"?   Richard Van Frank
• Needed: E. coli cDNA library   Bart Frank
• electroporation of primary spleen cells   Will Fuller
• In vitro transcription/translation   Will Fuller
• Pl Hirt procedure again   Patil J G
• skim milk agar   GS
• Chromosome painting   Gahtani
• <no subject>   Gahtani
• FISH multicolour background   Gahtani
• FISH multicolour background   Gahtani
• FISH multicolour background   Gahtani
• FISH multicolour background   Gahtani
• FISH multicolour background   Gahtani
• POST-DOCTORAL POSITION AVAILABLE   Olivier Gandrillon
• RNA EXO-nulcease   Dr. Peter Gegenheimer
• Site-Directed Mutagenesis   Rani Geha
• quikchange question   Rani Maurice Geha
• Running DNA on acrylamide gel   Rani Maurice Geha
• lactamase inhibitor?   Szatmari George
• Blunt-end troubles   Brenda Gerhardt
• yeast 2 hybrid screen on heart cDNA library   Mohammad Ghahremani
• Protein to predict ?   Indira Ghosh
• oligo biotinylation?   Kevin Gilbride
• Help gels cracking   Kevin Gilbride
• Needed: Rat spinal cord cDNA library!   Clemens Gillen
• sequencing problems w/ ALF II automated sequencer   Gizbab
• automated sequencing   Steven Goldberg
• Q. regarding rat liver NADPH-cytochrome P450 reductase   Steven Goldberg
• Troubleshooting PCR: smears   Steven Goldberg
• Cloning with Tth 111 I   Jose Gonzales
• Nuclease-free water   James Good
• organic P determination..   Frank R. Gorga
• 293 cells with transformed phenotype?   Lloyd Graham
• Summary of Re:293 cells with transformed phenotype   Lloyd Graham
• autofluorescence   Richard P Grant
• Asymmetric PCR   Sam Gray
• DNA Help   Annel Greene
• Nylon Membrane Troubles   GriffCouch
• Nylon Membrane Troubles   GriffCouch
• Sequencing and sharkteeth   Mike Gruidl
• gel doc/photo imaging system   Toumy Guettouche
• Semi-Dry Transfer Apparatus Reccomendations   Toumy Guettouche
• Where to buy NP-40? (Not Sigma)   Toumy Guettouche
• gel shift assay   Toumy Guettouche
• cross-linker   Toumy Guettouche
• Peroxidase-based chemiluminescence - best choice?   Toumy Guettouche
• pwo polymerase   HM
• Wanted: 18S or 28S probe   HY
• Seeking explanation on transformation results   HY
• Free molbio prg. available here !   HY
• Histological Stains   Kirk Hahn
• Centricon concentrator re-use   Anne E. Hall
• Ribonuclease protection assay - fluorescent ribonucleotides   Geoff Hall
• PCR fidelity (Taq error)   Matthew P. Hare
• protein phosphorylation   Dimitri Harmegnies
• Q: PCR cloning   Dimitri Harmegnies
• How to get rid of extra A added by Taq   Dimitri Harmegnies
• GST Fusion Proteins   Dick Haugland
• western blot   Alix Helme-Guizon
• western blot   Alix Helme-Guizon
• Question DNA isolation from seaweeds   Wieger Hemrika
• Pointer to FAQ list   Paul N Hengen
• What is Decon 90?   Paul N Hengen
• UV crosslinking   Paul N Hengen
• Semi-Dry Transfer Apparatus Reccomendations   Paul N Hengen
• Toothpicks?   Paul N Hengen
• :please maximise information in your email subjects   Paul N Hengen
• Designing Hairpin Structures in Oligos   Paul N Hengen
• Best way to search the 'net for reagents   Paul N Hengen
• Seeking gyrA462 CcdR E coli mutant   Paul N Hengen
• Designing Boomerang Primers   Paul N Hengen
• agarose gel electrophoresis   Paul N Hengen
• DNA extraction from cooked chicken and shrimp   Paul N Hengen
• native e. coli lysis/ inexpensive sonicator supplier source?   Paul N Hengen
• Semi-Dry Contact Transfer Method   Paul N Hengen
• [Q] DNA elution from agarose gel   Paul N Hengen
• possible contamination of Taq-polymerase with plasmid sequences   Paul N Hengen
• How to get rid of extra A added by Taq   Paul N Hengen
• matrix for spin columns   Paul N Hengen
• Minéral Oil - PCR   Paul N Hengen
• matrix for spin columns   Paul N Hengen
• Primers annealing protocol   Paul N Hengen
• Adding guanosine to your agarose?   Paul N Hengen
• Blue Colony?   Paul N Hengen
• test, ignore   Your Name Here
• Error bar in Microsoft Excel 5.0?   Guy Hermans
• Duffy Antigen Monoclonal?   Mike Herron
• acridine orange stain of DNA   Robert Hindges
• Lipofectin mediated delivery of oligo   Hung-yiu Ho
• Q. electroporation protocols wanted   Michael Hodges
• tempest on a toothpick   Susan Hogarth
• G-banding mouse spreads   Susan Hogarth
• dissolving of RNA   Susan Hogarth
• dissolving of RNA   Susan Hogarth
• Looking for Gel analysis software for Mac   Aki Hoji
• Chemiluminescence and Phosphoimager   Aki Hoji
• Semi-Dry Transfer Apparatus Reccomendations   Louis Hom
• test do not open   Hontie
• Are PCR reactions stable at 4 degrees?   Jan Hoolwerf
• skim milk agar   Jan Hoolwerf
• skim milk agar   Jan Hoolwerf
• nested-PCR?   Jan Hoolwerf
• DNA Auto-sequencing   Ao-lin Hsu
• How to precipitate the DNA   Ao-lin Hsu
• Help is needed for FSTAT program   Bixing Huang
• ANNOUNCEMENT: FEBS advanced course: frontiers of protein structure prediction 1997   Tim Hubbard
• ANNOUNCEMENT: have you a protein to predict? Call for prediction targets   Tim Hubbard
• ANNOUNCEMENT2: have you a protein to predict? Call for prediction targets   Tim Hubbard
• ANNOUNCEMENT2: FEBS advanced course: frontiers of protein structure prediction 1997   Tim Hubbard
• What is a "trypsinizing flask"?   Susan Hubchak
• lipid transfection reagent recipe needed   Mark Hughes
• PCR fidelity (Taq error)   Gene Huh
• Paraformaldehyde and autofluorescence.   IMMUNO-SHERBROOKE
• IRST Needs your help.   IRST
• oligo biotinylation?   Gabor Igloi
• Fifth Annual Nucleic Acid-Based Technologies   Cambridge Healthtech Institute
• skim milk agar   Irka
• Comments of usage of Dynabeads in sequencing PCR products   Pollanen Raimo J
• Plasmid restrictions cease working?   JLCruise
• organic P determination..   ANDY JOHNSTON
• detection of proteins in FPLC   ITABAJARA DA SILVA VAZ JUNIOR
• thomas scientific site?   ITABAJARA DA SILVA VAZ JUNIOR
• hot glass plates from acrylamide gels   Timothy R Jackson
• Are PCR reactions stable at 4 degrees?   Nick Jacobsen
• Whole genome amplification?   Nick Jacobsen
• hot glass plates from acrylamide gels   Nick Jacobsen
• Gas permeability meter   JanCzek
• Description of O2/CO2 Respirometer   JanCzek
• DNA purification from mouse tails   Teis Jensen
• WANTED: c-Myc construct to make expression vector   Dan Johnson
• HELP! sequencing gel problem   Donald Johnston
• cpm vs. dpm ?   Guenter Jost
• Yeast nucleus staining   Chong Jue
• pETor pGEX expression => opinions requested.   Chris Kafer
• autofluorescence   Bill Kalionis
• nested-PCR?   Huang Ke-xue
• Corex Tubes   Karen Kedzie
• Methicillin suppliers?   Nigel Kenward
• in situ hybridization   Gabriele Kerber
• Rotor compatibility??   Obaid Y. Khan
• Oligo Vital Stats   Chong-Yee Khoo
• Question!   Kim, Cheon
• [Q] DNA elution from agarose gel   Kim, Cheon
• Adhere cells onto slides for immunostaining   Dima Klenchin
• Peroxidase-based chemiluminescence - best choice?   Dima Klenchin
• Peroxidase-based chemiluminescence - best choice? - SUMMARY   Dima Klenchin
• Plant tissue PCR   Volker Knoop
• Free molbio prg. available here !   Rolf Kocherhans
• Free molbio prg. available here !   Rolf Kocherhans
• Free molbio prg. available here !   Rolf Kocherhans
• Free molbio prg. available here !   Rolf Kocherhans
• Bone morphogenic protein   Richard Koepsel
• Tall Lab Coats?   John Korte
• cDNA protocols   John Korte
• gel sticking anomoly   John Korte
• sodium acetate in sequencing gel buffers!   Alexander Kraev
• phosphatase question (library construction)   Alexander Kraev
• What's your favorite protocol book?   Alexander Kraev
• UV crosslinking   Alexander Kraev
• quikchange question   Alexander Kraev
• Seeking explanation on transformation results   Alexander Kraev
• Chemiluminescence and Phosphoimager   Alexander Kraev
• Cycle sequencing anyone?   Alexander Kraev
• matrix for spin columns   Alexander Kraev
• Q: DIG Chem-link   Alexander Kraev
• Ion Exchange Chro/HPLC with 6M Guanidine or Some Detergent?   Cornelius Krasel
• ORF without a particular amino acid ???   Cornelius Krasel
• What's your favorite protocol book?   Cornelius Krasel
• pET   Cornelius Krasel
• GST-fusion protein breakdown   Cornelius Krasel
• Anti-serine phosphatase   Gary Krause
• membrane proteins   Stefan Krautwald
• looking for p38 MAPK inhibitor   Stefan Krautwald
• looking for p38 MAPK inhibitor   Stefan Krautwald
• gel shift assay   Joachim Kremerskothen
• gel shift assay   Joachim Kremerskothen
• PCR-Primer degradation ??   Florian Kreppel
• DNA with RNA   Georg Kroeger
• possible contamination of Taq-polymerase with plasmid sequences   Georg Kroeger
• Cycle sequencing anyone?   Jerry Kropp
• differentiating between genomic & plasmid DNA   Scott Kuhns
• differentiating between genomic & plasmid DNA   Scott Kuhns
• In vitro transcription with T7 RNAP   Sami K J Kukkonen
• NP-40 Life?   Alexander N. Kukushkin
• ampicillin analog?   Alexander N. Kukushkin
• Metabolic labelling   L.Kempster
• quantitative gel-shift assays   martin LEACH
• Tris-Tricine protocols   KLAUS LEHNERT
• Troubleshooting PCR: smears   Baylor Molecular Genetics Lab
• Phosphorothioate primers in long PCR?   John Ladasky
• NP-40 Life?   John Ladasky
• What makes a good mammalian 3'UTR?   John Ladasky
• DNA fingerprinting mismatch   John Ladasky
• Sedimentation of Splicing stuff!   Michael Ladomery
• A BiaCore-like system   Olli Heikki Laitinen
• exact test for hardy-weinberg-equilibrium   Udo Landwehr
• How to separate vegf dimer into monomer?   Jacob Larsen
• How to separate vegf dimer into monomer?   Jacob Larsen
• Glycogen Synthase info needed...   Edouard Lauzier
• Hormonal reagents   Andrea LeBlanc
• How to clone a 20 bp fragment?   K van der Lee
• pET   Lee, Sun
• Looking for Gold Biotechnology   Nicolas Lemee
• transient transfection rat-1 cells   Rob Leurs
• Selecting human T cells by TCR V-beta   David N. Levy
• RT-PCR HELP!   French A. Lewis
• I got 870-bases reading data per run from ABI373 tonight!   Li, Der-Shiang
• ABI DNA sequencer--wanted   Li, Der-Shiang
• Yeast nucleus staining   Michael Lichten
• antibodies wanted   Ailing Lie
• test   Ailing Lie
• Yeast nucleus staining   Martin Ligr
• Fluorescent nuclear stains   Martin Ligr
• Online dictionary/encyclopedia   I-Kai Frank Lin
• ligation and UV   Zhanglin Lin
• LB media tablets   Henning Lindeberg
• Repeat Expansion Detection   Kimberly P. Lindsey
• ligase chain reaction   Nancy Linford
• E. coli genotypes   Nancy Linford
• E. coli strain MC1061/p3   Nancy Linford
• Crosslink in a parallel rather than anti-parallel manner?   Stephen Linhart
• PAGE sticking to both plates during seperation   Eric W. Linton
• What is Decon 90?   David C. Logan
• membrane protein prep   David C. Logan
• (no subject)   Jose Miguel Lopez
• Pipettor opinions   LouPass
• EtBr staining of DNA   LouPass
• Cloning with Tth 111 I   LouPass
• western blot   LouPass
• Centricon concentrator re-use   xianghuai Lu
• Clontech MatchMaker Library problem   Ray Lu
• Clontech MatchMaker Library problem   Ray Lu
• Comments on Novogen tagging systems   Mr. M.J. Lush
• Spodoptera frugiperda source   John G. Luz
• T7 select phage display   Jennifer Lyon
• Antisense transcripts   Jennifer Lyon
• T7 select phage display   Jennifer Lyon
• pET   Jennifer Lyon
• Immunoglobulin chimera between Heavy chain and protein   Jennifer Lyon
• Where to buy NP-40? (Not Sigma)   M. P. Frosch, MD, PhD
• Best way to search the 'net for reagents   margaret koziel MD
• 5PrimeRACE-noway?   FABIAN MOEBIUS
• Who has been coating glass with Streptavidin?   Dr. Michael T. MacDonell
• Amm.sulphate precipitation of protein in 6M urea   Malini Madiraju
• in situ and immunohistochemsitry   Mailer-Daemon at WELCHLINK.WELCH.JHU.EDU
• dissolving of RNA   Frank Maiwald
• dissolving of RNA   Frank Maiwald
• UV crosslinking   Rafael Maldonado
• Site-Directed Mutagenesis   Rafael Maldonado
• In vitro transcription/translation   Rafael Maldonado
• Bacterial DNA for PFGE   Rafael Maldonado
• Error bar in Microsoft Excel 5.0?   Jonathan B. Marder
• DNA with RNA   Mozart de Azevedo Marins
• Northern with 40mer oligo   Patrice Marion
• skim milk agar   Roy Markussen
• western blot   S K Marquardt
• Help gels cracking   S K Marquardt
• Help gels cracking   Ed Marsden
• plamid screening   Ed Marsden
• Cycle sequencing anyone?   Ed Marsden
• 4% Agar?   Rolf Marteijn
• PCR cloning   Rolf Marteijn
• Urea-containing buffers: How long can I store them?   Viraj Master
• Centricon concentrator re-use   Matthew L. Brown, Ph.D
• plasmid screening   Matthew L. Brown, Ph.D
• Circular dichroism   Gunter Maubach
• Methicillin suppliers?   J. Owen McCall
• BALB/c genomic libraries   Karina McQueen
• Plant Molecular Biology Post-Doctoral Position Available   Thomas Merchant
• Primers annealing protocol   Pascal Mertens
• (no subject)   Karen Metcalfe
• Membrane protein prep   Dr. David J. Meyer
• sodium acetate in sequencing gel buffers!   David Micklem
• EtBr staining of DNA   David Micklem
• matrix for spin columns   David Micklem
• EtBr staining of DNA   Mike
• Error bar in Microsoft Excel 5.0?   William A. Mitchell
• Blunt-end troubles   Barry Mook
• Help gels cracking   Mr. G. Morley
• Thanks! (was help gels cracking)   Mr. G. Morley
• Flow cytometry of transiently-transfected COS cells   Kevin A. Mulcahy
• Amplitaq Gold - How do they do that???   Gordon Munro
• FISH multicolour background   Gordon Munro
• Which strand does T7 RNA pol transcribe from?   Gordon Munro
• Effect of NaOH on biotin-streptavidin??   Gordon Munro
• DNA purification from mouse tails   Bernard Murray
• ampicillin analog?   Bernard Murray
• agarose gel electrophoresis   Bernard Murray
• Wanted: 18S or 28S probe   Bernard Murray
• Q: solublility of CsCl in EtOH and iPropOH? (recovery of plasmid   Bernard Murray
• tempest on a toothpick   Tsunehisa Namba
• Blunt-end troubles   Darren Natale
• Best way to search the 'net for reagents   Darren Natale
• matrix for spin columns   Darren Natale
• How to use BLAST   Mauricio Neira
• Screening lambda gt11 library   Mauricio Neira
• RNazol - where to get it?   Mauricio Neira
• How to get rid of extra A added by Taq   Mauricio Neira
• sodium acetate in sequencing gel buffers!   Chia Jin Ngee
• Hirt procedure pl.   Rick Nicholson
• gel doc/photo imaging system   Rick Nicholson
• EtBr staining of DNA   Gerd Nilsen
• ampicillin analog?   Gerd Nilsen
• How to re-amplify the DDRT-PCR band?   Gerd Nilsen
• Removal of White Blood Cells   Delvac Oceandy
• Sequence motifs on antisense strand   Martin Offterdinger
• B-M Titan RT-PCR   Martin Offterdinger
• Pipettor opinions   J. Oliver
• NP-40 Life?   The Kindly One
• Extraction of DNA   Paul Oxberry
• 2-hybrid system problem (Clontech).   PC-BS-09
• TRAP ASSAY   June Eva Paciga
• bioinformatics and virology course in Cuba   Alejandro Palacios
• Help gels cracking   Pascal_Bochet
• 96-wells PCR plates sealing   Stephane Pasteau
• Q: anti-phosphothreonine/serine Abs for immunoprecipitation   John Pawlowski
• Afinity purification with MAb - kits, protocols?   D. Pearton
• IgG conc in ascites?   D. Pearton
• Sequencing large inserts??   Silvia Peneula
• lactamase inhibitor?   Tom A. Penfound
• nuclear encoded mitochondrial proteins   Graziano Pesole
• Blunt-end troubles   Pete
• 96-wells PCR plates sealing   Pete
• UV crosslinking   Pete
• RNazol - where to get it?   Niels Erik Petersen
• Adenovirus type 2 DNA   Phillip T. Moen, Jr.
• Ion Exchange Chro/HPLC with 6M Guanidine or Some Detergent?   John Philo
• gel shift assay   William K. Pierce
• How to clone a 20 bp fragment?   William K. Pierce
• Please help contact Amsterdam Red Cross   Eva Pietrzak
• Medarex, Annandale   Eva Pietrzak
• T7 or SP6 RNA Pol promoter sequence   Mircea Podar
• precipitating nucleotides protocol   Mircea Podar
• Source for Ab needed   Eyck Poetter
• degenerate PCR   Robert K. Pope
• Opinions Statagene hot top robocycler   Andrei Popov
• What is a "trypsinizing flask"?   Michael B. Porter
• Nylon Membrane Troubles   Michael J. Prigge
• NEN renaissance kit   Michael J. Prigge
• automated sequencing   Kiley R. Prilliman
• FISH probes for androgen receptore?   Hans-Bernd Prisack
• GST Fusion Proteins   Michael Quail
• questions about protein purification equipment   RJSamples
• RT-PCR HEEELP!   Marta Radman-Livaja
• Postdoctoral position available   Ramesh Raina
• plamid screening   Srikumar M. Raja
• DNA extraction from cooked chicken and shrimp   Om Rajora
• Amino Acids Stock Solutions   Karthi Ramachandran
• In-gel Raf assay   Alberto Ramirez
• Blunt-end troubles   Colin Rasmussen
• Blunt-end troubles   Colin Rasmussen
• Gibco AFLP kit   Martha L. Reed
• transient transfection of RAW264.7 macrophages   Christian Reiser
• Opinions Statagene hot top robocycler   Mary Remington
• How to re-amplify the DDRT-PCR band?   Mary P. Remington
• B-M Titan RT-PCR   famille Renard
• cDNA protocols   Robert Pomponio, Ph.D.
• pre-made Northern blots   Julie Roberts
• Tall Lab Coats?   Julie Roberts
• Wanted: 18S or 28S probe   Richard Rohan
• Nucleotide precipitation protocol   Sean Rollins
• Nucleotide precipitation protocol   Sean Rollins
• precipitating nucleotide protocol   Sean Rollins
• precipitating nucleotides protocol   Sean Rollins
• run-off transcription   Sean Rollins
• teflon tissue grinders   Hiranya Roychowdhury
• sodium acetate in sequencing gel buffers!   Hiranya Roychowdhury
• Q: SAP (Shrimp alkaline phosphatase) at 4degC   Hiranya Roychowdhury
• Screening lambda gt11 library   Hiranya Roychowdhury
• Running DNA on acrylamide gel   Hiranya Roychowdhury
• [Q] DNA elution from agarose gel   Hiranya Roychowdhury
• DNA extraction from cooked chicken and shrimp   Zbigniew Rudzki
• PCR cloning   Monica Ruiz-Noriega
• agarose gel electrophoresis   Andreas Rump
• sequencing problems   Connie Runyan
• Commercially avail. pathogen DNAs   RussVG
• Yeast centromere element sequences   Peter Russell
• Help gels cracking   George Rutherford
• E. coli dam- Strain Needed   Steve Ruttenberg
• (no subject)   Kelley Astrid Ruud
• EtBr staining of DNA   Ed Rybicki
• western blot   Ed Rybicki
• Semi-Dry Transfer Apparatus Reccomendations   Ed Rybicki
• determining titer   Ed Rybicki
• Antibodies against mouse gelatinases A and B   S.Mentzel at PATHOL.AZN.NL
• Help :Photobiotin & probe   Verasak Sahachaisaree
• Tli polymerase for PCR...   Sai
• PCR cloning   Sai
• hot glass plates from acrylamide gels   Klaus Salger
• HELP! sequencing gel problem   Klaus Salger
• Help: In gel Hybridization Technique   Karen Samis
• Help: In gel Hybridization Technique   Karen Samis
• ribonuclease protection assay and non-isotopic label   Lennie Samsell
• problems with GST-peptide binding after Sarkosyl lysis   P.J. van Santbrink
• automated sequencing   Paulo Santos
• A little help here...   Hany Saqr
• A little help here...   Hany Saqr
• western blot   Hany Saqr
• RNA EXO-nulcease   David Sarracino
• test   Stephen Saul
• probe labelling for non-radioactive in situ hybridization   Stephen Saul
• Kidney glomeruli mRNA prep. ?   Heinz-Juergen Schaefers
• Sizes of 28S, 18S & 7S rRNA   Heinz-Juergen Schaefers
• western blot   Heinz-Juergen Schaefers
• a device to measure whole plant fluorescence   Wolfgang Schechinger
• how do restriction endonuclweases work?   Wolfgang Schechinger
• EtBr staining of DNA   Wolfgang Schechinger
• Online dictionary/encyclopedia   Wolfgang Schechinger
• Afinity purification with MAb - kits, protocols?   Wolfgang Schechinger
• :please maximise information in your email subjects   Wolfgang Schechinger
• 4% Agar?   Wolfgang Schechinger
• NP-40 Life?   Wolfgang Schechinger
• Q: SAP (Shrimp alkaline phosphatase) at 4degC   Wolfgang Schechinger
• Q: solublility of CsCl in EtOH and iPropOH? (recovery of plasmid   Wolfgang Schechinger
• (no subject)   Jennifer Schmidt
• Library Construction Advice: Cosmid vs. Electroporation   Robert A. Schoenfeld
• FMC -long ranger and ABI 373A   Bernhard Schu
• T7 or SP6 RNA Pol promoter sequence   Joern Schwede
• FISH multicolour background   Geoff Scopes
• ligase chain reaction   Geoff Scopes
• 2bp Ladder   Geoff Scopes
• Probe purification   Brendan Scott
• E. coli strain MC1061/p3   John Richard Seavitt
• TRAP assay   Loryn Sellner
• H. simplex TK cDNA wanted   Jurgen Seppen
• Phage DNA RAPD-PCR   Fernando Sesma
• quantitative gel-shift assays   P.J. Shaw
• MacConkey instead of XGAL ?   Glen Shearer
• Membrane protein prep   Shinhan Shiu
• autofluorescence   Shinhan Shiu
• Antibodies to human MnSOD   Linda M Siemankowski
• Which companies provide pATH?   Sineenat Siri
• PAGE sticking to both plates during seperation   Slappy
• Urea-containing buffers: How long can I store them?   Dr Koen A.L. De Smet
• Help gels cracking   Wendy-Anne Smith
• agarose gel electrophoresis   Kimberley Snowden
• Problems with biolistics protocol   Kimberley Snowden
• agarose gel electrophoresis   Rod Snowdon
• How to get rid of extra A added by Taq   Xinyu X Song
• Antibodies against mouse gelatinases A and B   Keld Sorensen
• western blot   Keld Sorensen
• desperately need help   Keld Sorensen
• Afinity purification with MAb - kits, protocols?   Keld Sorensen
• western blot   Keld Sorensen
• Free molbio prg. available here !   Keld Sorensen
• please maximize information in your email subjects   Keld Sorensen
• dissolving of RNA   David F. Spencer
• dissolving of RNA   David F. Spencer
• Subject: Q: solublility of CsCl in EtOH and iPropOH? (recovery of plasmid   Dom Spinella
• Meeting   Tim Stabler
• desperately need help   Bob Steinberg
• Semi-Dry Transfer Apparatus Reccomendations   Bob Steinberg
• Chemiluminescent Southern Blots   Bob Steinberg
• Metabolic labelling   Bob Steinberg
• Urea-containing buffers: How long can I store them?   Bob Steinberg
• Which strand does T7 RNA pol transcribe from?   Peter Stoilov
• skim milk agar   Tony Stott
• Software for RNA 2-D structure analysis   Pierluigi Strippoli
• Looking for Gold Biotechnology   Sailesh Surapureddi
• screening library   Caroline Szymeczek-Seay
• Coverslip-base culture vessels   Caroline Szymeczek-Seay
• EtBr staining of DNA   Caroline Szymeczek-Seay
• determining titer   Caroline Szymeczek-Seay
• probe specific activity   Caroline Szymeczek-Seay
• 293 cells with transformed phenotype?   Caroline Szymeczek-Seay
• Screening lambda gt11 library   TLANE at watson.princeton.edu
• pre-made Northern blots   Kohta Takahashi
• pre-made Northern blots   Kohta Takahashi
• dna array   Seng-Lai Tan
• Semi-Dry Transfer Apparatus Reccomendations   Ron Tate
• Help gels cracking   Ron Tate
• ppt. Small RNA   Matt Thomas
• Who has been coating glass with Streptavidin?   Matt Thomas
• X-linker ratio in Acrylamide gels   Matt Thomas
• antibody against polyoma LT   Tim
• comments about kjeldhal method   Enrique Toledo
• Seeking gyrA462 CcdR E coli mutant   Bradley Turner
• Epitope Tagging Questions...   Simon Twigger
• Looking for Gel analysis software for Mac   Darren Tyson
• western blot   Darren Tyson
• Heteroduplex formation   de Jesus . Vicente V.
• help for reducing sugar analysis   D. Vadnais
• angiotensin II type I receptor   Steeve Veronneau
• QE: nuclear extract   Dr.Tilman Voss
• QE: nuclear extracts   Tilman Voss
• Boiling RNA   Jim WIck
• skim milk agar   Kimberly Walker
• Urea-containing buffers: How long can I store them?   Stefan Walter
• DNA purification from mouse tails   John Watson
• Blunt-end troubles   John Watson
• BALB/c genomic libraries   John Watson
• gel shift assay   John Watson
• Storing RNA in formamide: The Downside?   Chi-kuang Wen
• YeastExpressionVector   Marcus Wernitz
• in situ hybridization   William
• Error bar in Microsoft Excel 5.0?   Alan Williams
• gel doc/photo imaging system   Ann L. Williams
• Transfection of mammalian cells   Tori D. Williams
• Detergents function??   Randy Willis
• pETor pGEX expression => opinions requested.   Randy Willis
• Comments on Novagen tagging systems   Randy Willis
• Where to buy NP-40? (Not Sigma)   Randy Willis
• Testing Please Ignore   A Wilson
• 2nd RFD: sci.bio.immunocytochem   A Wilson
• chromosome fixative   Mark R Wilson
• cpm vs. dpm ?   Troels Wind
• agarose gel electrophoresis   David Winterbourne
• assay for neuromelanin   Graeme Wistow
• Troubleshooting PCR: smears   Harry Witchel
• Are PCR reactions stable at 4 degrees?   Harry Witchel
• 4% Agar?   Harry Witchel
• PCR   Kenneth KangWa Wong
• PCR   Kenneth KangWa Wong
• Anti-serine phosphatase   Jim Woodgett
• cDNA for angiotensin II receptor type I   Woong Choi,M.D.
• angiotensin II type I receptor   Woong Choi,M.D.
• E. coli strain MC1061/p3   Woong Choi,M.D.
• help for e-mail address   Woong Choi,M.D.
• screening library   Mayumi Yagi
• [Q] Where is the software ?   Hisanori Yamamoto
• MJ Research PTC-200 interchangable block   Xie Yi
• western blot   Rupert Yip
• Anti-serine phosphatase   Ian A. York
• Scanners   Ian A. York
• Flow cytometry of transiently-transfected COS cells   Ian A. York
• 293 cells with transformed phenotype?   Ian A. York
• Help gels cracking   Ian A. York
• E. coli strain MC1061/p3   Ian A. York
• Blunt-end troubles   T.J. Young
• western blot   David Tak Yan Yu
• membrane proteins   Jelena Zaitseva
• EtBr staining of DNA   Marieke R. Koedood Zhao
• 2-hybrid system problem (Clontech).   Marieke R. Koedood Zhao
• Help gels cracking   Marieke R. Koedood Zhao
• Can Agrobacterium live in plants and pass from parent to progeny?   Yuanxiang Zhou
• Seeking explanation on transformation results   Yuanxiang Zhou
• Where to get VirG and VirE antibodies   Yuanxiang Zhou
• Adenovirus expressing T7RNAP   Christian Zuppinger
• looking for multiple-Nucleic Acid sequences alignment software for PCs   ahybio at shell.scsti.ac.cn
• Anti-serine phosphatase   hadi al-hasani
• Epitope Tagging Questions...   hadi al-hasani
• lipid / liposome administration in vivo   alena at scientia.up.ac.za
• in vivo administration of lipid components by LIPOSOMES   alena at scientia.up.ac.za
• in vivo administration of lipid components by LIPOSOMES   alena at scientia.up.ac.za
• Semi-Dry Transfer Apparatus Reccomendations   eric anderson
• What's your favorite protocol book?   eric anderson
• ampicillin analog?   eric anderson
• Peroxidase-based chemiluminescence - best choice?   eric anderson
• Transfection of mammalian cells   eric anderson
• matrix for spin columns   eric anderson
• PCR cloning   eric anderson
• Dissociating agents to prevent protein aggregation   antonio at liverpool.ac.uk
• How to search GenBank for ORF without a particular amino acid ???   bb1dopeg
• ORF without a particular amino acid ???   bb1dopeg
• Commercially cut cosmid DNA   billing at vetsci.microvet.arizona.edu
• In vitro transcription with T7 RNAP   brett
• Which strand does T7 RNA pol transcribe from?   brett
• Lipofectin mediated delivery of oligo   brett
• RNase-free Glycogen Solution   brett
• run-off transcription   brett
• Epitope Tagging Questions...   brett
• Q: anti-phosphothreonine/serine Abs for immunoprecipitation   bs133 at columbia.edu
• License to field release   chtsai at MTU.EDU
• anti-c and anti-f supplier informtion   cmpng4vr at aol.com
• ppGpp Source?   crawford at bscr.uga.edu
• detection of proteins in FPLC   bipin k. dalmia
• Adenovirus type 2 DNA   dalton at neb.com
• Pectin rich / RNA   dao at bio.grenet.fr
• Blue Colony?   ddsyr at NS.EAST.CN.NET
• preferential amplification by proofreading Taq ???   ed
• preferential amplification by proofreading Taq ???   ed
• Perkin-Elmer DNA Thermal Cycler- HP ThinkJet Printer?   ericsson at acsu.buffalo.edu
• Perkin-Elmer DNA Cycler to Macintosh?   ericsson at acsu.buffalo.edu
• How to re-amplify the DDRT-PCR band?   fbedin
• gel doc/photo imaging system   gavin at molgennn.demon.co.uk
• What's your favorite protocol book?   gdavis at ou.edu
• looking for Gold Biotechnology   paul gold
• FISH probe random prime or nick translate   paul gold
• Reference for mouse and human codon frequency   paul gold
• Cheapest "clean" Taq polymerase?   graham at biodec.wustl.edu
• Nylon membrane lot & false positive colony lift?   graham at biodec.wustl.edu
• RT-PCR on formalin fixed paraffin sections   hcorbett at garnet.berkeley.edu
• phage titration   hrichter at ulys.unil.ch
• universal standard?   xudong huang
• dissolving of RNA   xudong huang
• dissolving of RNA   xudong huang
• DNA fingerprinting mismatch   icas at sympatico.ca
• Random peptide library   ijiwaru at wheel.dcn.davis.ca.us
• M13 glucose repression   ijiwaru at wheel.dcn.davis.ca.us
• Single Copy Plasmids   daniel jacobs
• ppt. Small RNA   daniel jacobs
• G+C DNA Analysis   daniel jacobs
• Question about DM1 bacteria strain.   jh7 at acsu.buffalo.edu
• SDS effect ?   jing at NIC.HBU.EDU.CN
• sequence data anomaly-PCR clone   jmorrey
• ribosome 3D structure   kasper at kemi.aau.dk
• Southern Blotting of Candida albicans chromosome   SungHoon kim
• heat-shock proteins cDNA and AB   stanislav kopriva
• detection of IP3 production   lab at PUBLIC.EAST.CN.NET
• Adaptor construction   e. lader
• Apparati for Northern Blotting   e. lader
• ribonuclease protection assay and non-isotopic label   e. lader
• Adaptor construction   charles lawrie
• Streptavidin protocol?   lbloch
• antibodies against ß-glucuronidase in parafin sections?   luc
• nested-PCR?   ma
• Direct or Nested PCR?   ma
• GST-fusion protein breakdown   marcvdc at lmb1.rug.ac.be
• Pwo polymerase blues   hassane mchaourab
• western blot   mfm### at rzmail.uni-erlangen.de
• UV crosslinking   miketrower at aol.com
• ampicillin analog?   mstanbro at bih.harvard.edu
• cDNA probe preparation by PCR   your name
• Detergents function??   nestgrp at earthlink.net
• Ion Exchange Chro/HPLC with 6M Guanidine or Some Detergent?   newera at plaza.snu.ac.kr
• How to re-amplify the DDRT-PCR band?   nhwu at PUBLIC3.BTA.NET.CN
• what is what I need   nhwu at PUBLIC3.BTA.NET.CN
• RNase-free Glycogen Solution   nhwu at PUBLIC3.BTA.NET.CN
• phage titration   noguei01 at mcrcr6.med.nyu.edu
• troubles with antibiotic   Namy olivier
• Urea-containing buffers: How long can I store them?   packard at kersur.net
• Protein Sequence Analysis ( Shareware )   palmeric at erols.com
• Growing Yeast @ 37 degrees? Help   steven petros
• Pvu II partial digest conditions needed   plxsrt at pln1.nott.ac.uk
• Thanks (silver staining)   r.l.bates
• antibody purification   r.l.bates
• HELP! sequencing gel problem   s.pritchard
• Help gels cracking   s.pritchard
• Comments on Novogen tagging systems   sai at uab.edu
• endothelial cell cDNA Library for two hybrid system   shussain
• Single Copy Plasmids   someone
• sodium acetate in sequencing gel buffers!   cynthia stryker
• Looking for Gold Biotechnology   cynthia stryker
• Cheapest Oligos Results   cynthia stryker
• Cheaper Trizol Alternative?   cynthia stryker
• Free DNA Purification Kit   techmen at technologymentors.com
• PCR / DNA Purification Kit   techmen at technologymentors.com
• REFERENCE MANAGEMENT SHAREWARES AVAILABLE?   wang at LTC.ADMWORLD.COM
• triton micelle size   wegloehner at mpimg-berlin-dahlem.mpg.de
• Coating Plates with streptavidin   yserov at medhla.dmed.iupui.edu
• mAbs against mouse gelatinases A and B?   xx yy
• sodium acetate in sequencing gel buffers!   zarir

Last message date: Mon Mar 31 21:35:10 EST 1997
Archived on: Sun Mar 6 21:24:05 EST 2005

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