Home bacterial incubator.

Farida Safadi-Chamberlain fsafadi at LAMAR.COLOSTATE.EDU
Thu May 1 16:01:37 EST 1997



Be very careful with the size of the box and the 25W lamp, a lamp in a box
has caused the box to heat and catch fire in a very similar incident.


On 1 May 1997, David L. Haviland, Ph.D. wrote:

> At 13:06 5/1/97 GMT, Robert  Cowherd wrote:
> 
> Robert:
> 
> >This is not a very important, nor wide spread problem but here goes...
> 
> I wouldn't say that at all...  public education is a an unwritten mandate
> in our graduate training.  If we can't educate John/Jane.Q.Public in
> science, how can we expect them to want to have science funded?
> 
> >I am helping a couple of eleven year olds to do a "science project."  I 
> >suggested that they do a study on the efficacy of hand washing and soap 
> >manufacturer's claims of being "antibacterial."  I've made some plates 
> >and tried a pilot run by touching the agarose then leaving the plates out 
> >on the counter.  Some 36 hours later there are one or two colonies.  
> >
> >Time is not real important but I can't carry plates to and fro from home 
> >(their's) to the lab on a daily basis.  Any ideas about a simple 
> >incubator?  I've thought about using the hot water heater as a heat 
> >source.  Perhaps the small area around it might approximate 37 degrees 
> >C.  Anyone else have an alternative?  Thanks.
> 
> Out of lazyness, I have conducted ligations and transformations on fridays,
> and simply left the plates on my bench at room temp till monday.  I see
> colonies just as I would had I left them in the 37'C incubator for 24 hours.
> 
> One idea, get a hold of a decent sized box, say 2-3 cubit feet.  Just put a
> 25W lamp in there.  That will warm it up above room temp but heat leakage
> from the box will likely prevent it going much above 35-ish.  It depends on
> how soon you  need to see results and how often the class meets.  The box
> with the lamp may very well yeild colonies within 24 hours.  (I often turn
> on the 60W bulb in my electric oven a hour before I put my bread in there
> to rise - works quite well, so I see no reason why a similar approach
> wouldn't work for growing bacterial colonies.)  I know, this is the methods
> sub, not rec.food.cooking!
> 
> Hope this helps,
> David
> 
> =============================
>  David L. Haviland, Ph.D.
>  Asst. Prof. Immunology 
>  University of Texas - Houston, H.S.C.
>  Institute of Molecular Medicine  
>  2121 W. Holcombe Blvd.  
>  Houston, TX  77030 
>  Internet:"dhavilan at imm2.imm.uth.tmc.edu" 
>  Voice: 713.500.2413  FAX: 713.500.2424
>  ------------------------------------------------------  
> " Sometimes you're the windsheild, sometimes you're the bug."
> =============================
> 
> 
> 




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