Home bacterial incubator.
fsafadi at LAMAR.COLOSTATE.EDU
Thu May 1 16:01:37 EST 1997
Be very careful with the size of the box and the 25W lamp, a lamp in a box
has caused the box to heat and catch fire in a very similar incident.
On 1 May 1997, David L. Haviland, Ph.D. wrote:
> At 13:06 5/1/97 GMT, Robert Cowherd wrote:
> >This is not a very important, nor wide spread problem but here goes...
> I wouldn't say that at all... public education is a an unwritten mandate
> in our graduate training. If we can't educate John/Jane.Q.Public in
> science, how can we expect them to want to have science funded?
> >I am helping a couple of eleven year olds to do a "science project." I
> >suggested that they do a study on the efficacy of hand washing and soap
> >manufacturer's claims of being "antibacterial." I've made some plates
> >and tried a pilot run by touching the agarose then leaving the plates out
> >on the counter. Some 36 hours later there are one or two colonies.
> >Time is not real important but I can't carry plates to and fro from home
> >(their's) to the lab on a daily basis. Any ideas about a simple
> >incubator? I've thought about using the hot water heater as a heat
> >source. Perhaps the small area around it might approximate 37 degrees
> >C. Anyone else have an alternative? Thanks.
> Out of lazyness, I have conducted ligations and transformations on fridays,
> and simply left the plates on my bench at room temp till monday. I see
> colonies just as I would had I left them in the 37'C incubator for 24 hours.
> One idea, get a hold of a decent sized box, say 2-3 cubit feet. Just put a
> 25W lamp in there. That will warm it up above room temp but heat leakage
> from the box will likely prevent it going much above 35-ish. It depends on
> how soon you need to see results and how often the class meets. The box
> with the lamp may very well yeild colonies within 24 hours. (I often turn
> on the 60W bulb in my electric oven a hour before I put my bread in there
> to rise - works quite well, so I see no reason why a similar approach
> wouldn't work for growing bacterial colonies.) I know, this is the methods
> sub, not rec.food.cooking!
> Hope this helps,
> David L. Haviland, Ph.D.
> Asst. Prof. Immunology
> University of Texas - Houston, H.S.C.
> Institute of Molecular Medicine
> 2121 W. Holcombe Blvd.
> Houston, TX 77030
> Internet:"dhavilan at imm2.imm.uth.tmc.edu"
> Voice: 713.500.2413 FAX: 713.500.2424
> " Sometimes you're the windsheild, sometimes you're the bug."
More information about the Methods