composite primers

Hallgeir Bergum hallgeir at mtsrv.mtfs.unit.no
Thu May 1 06:24:41 EST 1997


Hi! Netters

We have some problems with a linker primer we have made. The primers are
around 40 mers. We have tried with long denaturation time and low
annealing temp, but we dont get any bands. Hope some one could give us
some advises. The linker primers are made for generation of a cometetive
fragment for competetive rt-PCR.

Vidar



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