DNA isolation from Rhodococcus

Pyung Cheon Lee pclee at CAIS.KAIST.AC.KR
Thu May 1 02:40:03 EST 1997


Hi,

  Does anyone have a reliable protocol for isolation of chromosomal DNA 
and plasmid DNA from Gram positive bacteria, Rhodococcus species?

In my experiments with Rhodococcus, this cell have a tendency to adhere 
to each other and aggregate during culture.

So, it is difficult to resuspend the Rhodococcus cell pellet and lyse 
them with solution containing SDS.

Help me from this problems....


Thanks in advance !!!!


Pyung Cheon Lee

Department of chemical engineering,
KAIST,
South Korea.

E-mail: pclee at cais.kaist.ac.kr



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