DT40 chicken cell line
bbeitzel at jeeves.ucsd.edu
Fri May 2 14:05:12 EST 1997
Anybody ever used DT40 cells? I have just started trying to grow some and
have a few questions:
1) What medium do you grow them in?
2) If you use the medium recommended by ATCC
(DMEM,beta-mercaptoethanol, glutamine,chicken serum, fetal bovine serum,
and tryptose phosphate broth,) how do you prepare it? I tried to filter
500mls of it, and the filter plugged after 100mls.
Any help would be much appreciated.
Thanks in advance,
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