DT40 chicken cell line

Brett Beitzel bbeitzel at jeeves.ucsd.edu
Fri May 2 14:05:12 EST 1997


Anybody ever used DT40 cells?  I have just started trying to grow some and
have a few questions:

   1)  What medium do you grow them in?  
   2)  If you use the medium recommended by ATCC
(DMEM,beta-mercaptoethanol, glutamine,chicken serum, fetal bovine serum,
and tryptose phosphate broth,) how do you prepare it?  I tried to filter
500mls of it, and the filter plugged after 100mls.

Any help would be much appreciated.

Thanks in advance,



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