a. Transformation efficiency vs cell density
gnattras at waite.adelaide.edu.au
Fri May 2 20:19:24 EST 1997
When preparing competent E. coli the cell density is monitored using
OD600nm. I generally grow DH5a between 0.5-0.6. I've been using a
protocol by Inoue, 1990 (Gene). How do you determine the final
resuspension volume. How many viable cells/ml should the competent cells
be resuspended at?
Also, this method is supposed to produce competent cells returning
10/9 (10 to the 9) transformants. They used pBR322 as their
transformation control while I've used pBluescript. Does the plasmid
vector used have any effect on the transformation efficiency?
University of Adelaide
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