spermidine/spermine prep

Frederik Intelmann intelman at nospam.uni-hohenheim.de
Fri May 2 03:38:42 EST 1997

In article <33661E84.662 at em.agr.ca>,
   "John E. Hachey" <hachey at em.agr.ca> wrote:
>Can anyone tell me if it is O.K to autoclave
>spermidine od spermine. I can find about zero
>information on preparation and storage of
>solutions containing these compounds.

The publication on spermine precipitation of DNA by Hoopes and McClure 
(Nucleic acids rersearch Vol.9 (20) p. 5493-5504, 1981) advises storage "at 4 
°C for short term storage (weeks)" and "-20°C for long term storage" but says 
nothing about autoclaving such solutions. I autoclave my 0.1 M solutions and 
my precipitations still work, although I sometimes have to increase the time 
of the centrifugation step (20 instead of 10 minutes).

       Frederik Intelmann

Not "Thou shalt not". Say "I will not".
(Terry Pratchett, Feet of clay)

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