Overhang A of PCR
Anxo Vidal Figueroa
fsavidal at uscmail.usc.es
Fri May 2 08:50:26 EST 1997
On 28 Apr 1997, ma wrote:
> Dear netters
> Are proofread Taq enzymes like pfu and vent also add A in the pcr product end like
> usually Taq? I concern this because I want ligate the pcr products by T-vector. Thanks
> for your seggestion.
I think Vent Polymerase (New England Biolabs) does not add tails to the
products, so you get blunt-ended PCR products. You do not need a T-vector
to clone them but every plasmid with a blunt site.
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