Overhang A of PCR

Anxo Vidal Figueroa fsavidal at uscmail.usc.es
Fri May 2 08:50:26 EST 1997



On 28 Apr 1997, ma wrote:

> Dear netters
>  Are proofread Taq enzymes like pfu and vent also add A in the pcr product end like
>  usually Taq? I concern this because I want ligate the pcr products by T-vector. Thanks
>  for your seggestion.
>  ma
> 
> 
> 
> 
I think Vent Polymerase (New England Biolabs) does not add tails to the 
products, so you get blunt-ended PCR products. You do not need a T-vector
to clone them but every plasmid with a blunt site.
Best Regards

Anxo



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