Shearing DNA

eric anderson e-anderson at ski.mskcc.org.nospam
Tue May 6 09:34:15 EST 1997


In article <Pine.A41.3.95.970505172855.32796D-100000 at aix1.uottawa.ca>, ed
<s535290 at aix1.uottawa.ca> wrote:

> Ian,
> 
> 
> why don't you try nebulizing the DNA ?  someone in this department uses
> nebulization very frequently and has his system finely tuned to generate a
> median fragment size of about 3 Kb. He uses it for making shotgun
> libraries for large scale sequencing. If you are interested, drop me a
> line and I can pass this person's e-mail address to you. The procedure is
> so foolproof that we used it on an undergraduate molecular biology lab :)
> ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^

actually, foolproof is when it works in the hands of a rotation grad
student _AND_ a postdoc.

eric

>

-- 
Eric C. Anderson
Memorial Sloan-Kettering Cancer Center
Sloan-Kettering Institute
1275 York Ave. Box 470
New York, NY  10021
(212) 639-2977
e-anderson at ski.mskcc.org
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