PFU

daniellh at biovax.leeds.ac.uk daniellh at biovax.leeds.ac.uk
Tue May 6 09:02:36 EST 1997


PFU is better for cloning than Taq because of its proofreading ability.
It's quite hard to optimise your PCR conditions, and as mentioned
by another newsgroup subscriber, the extenion stages needs to be longer
then for taq (try 1 to 1 1/2 mins to start with). There should be no
more problems than usual getting ligation to work properly or 
transformation.
Dani.  Leeds University.



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