Shearing DNA

Bernard Murray bernard at
Wed May 7 00:02:47 EST 1997

In article <e-anderson-0605970934150001 at>, 
e-anderson at says...
>In article <Pine.A41.3.95.970505172855.32796D-100000 at>, ed
><s535290 at> wrote:
>> Ian,
>> why don't you try nebulizing the DNA ?  someone in this department uses
>> nebulization very frequently and has his system finely tuned to generate a
>> median fragment size of about 3 Kb. He uses it for making shotgun
>> libraries for large scale sequencing. If you are interested, drop me a
>> line and I can pass this person's e-mail address to you. The procedure is
>> so foolproof that we used it on an undergraduate molecular biology lab :)
>> ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^

>actually, foolproof is when it works in the hands of a rotation grad
>student _AND_ a postdoc.

Noooo....  The ultimate in foolproofness is when it can be done by
a visiting clinician (who doesn't try and delegate it to a nurse).
Overconfidence is a far greater problem than technical incompetence.
	Oh, I have some horror stories...

Bernard Murray, Ph.D.
bernard at (National Cancer Institute, NIH, Bethesda MD, USA)

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