Direction of Insert in T-Vector

plxsrt at pln1.nott.ac.uk plxsrt at pln1.nott.ac.uk
Tue May 6 05:27:57 EST 1997


prigge at darkwing.uoregon.edu (Michael J. Prigge) wrote:

>plxsrt at pln1.nott.ac.uk wrote:
>> ddsyr at NS.EAST.CN.NET (ma) wrote:
>> 
>> >checked four clones of transformants, I found they were all one direction. > 
>> it could still be by chance- 1/16 samples of four clones will be all
>> the same orientation by pure chance.  
>> 
>...or (2*1/16=) 1/8 for all in one or the other orientation.

Agreed, but  the bottom line is that if you want a clone in a
particular orientation  you should test more clones first and then if
necessary worry about the stranger things that might be happening in
your experiment.

Simon T


>Michael Prigge                          Phone: (541) 346-4256
>Institute of Molecular Biology            Fax: (541) 346-5891
>University of Oregon /\\ /\\ /\\ /\\ /\\ /\\ /\\ /\\ /\\ /\\ 
>Eugene, OR 97403   \\/ \\/ \\/ \\/ \\/ \\/ \\/ \\/ \\/ \\/ \\





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