Shearing DNA

David L. Haviland, Ph.D. dhavilan at IMM2.IMM.UTH.TMC.EDU
Wed May 7 11:34:30 EST 1997

At 09:34 5/6/97 -0500, eric anderson wrote:
>In article <Pine.A41.3.95.970505172855.32796D-100000 at>, ed
><s535290 at> wrote:
>> Ian,
>> why don't you try nebulizing the DNA ?  someone in this department uses
>> nebulization very frequently and has his system finely tuned to generate a
>> median fragment size of about 3 Kb. He uses it for making shotgun
>> libraries for large scale sequencing. If you are interested, drop me a
>> line and I can pass this person's e-mail address to you. The procedure is
>> so foolproof that we used it on an undergraduate molecular biology lab :)
>> ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
>actually, foolproof is when it works in the hands of a rotation grad
>student _AND_ a postdoc.


Umm... are you serious???  A nebulizer?   That noisy machine that
aerosolized albuterol for various members of my family when the pollens and
molds get too high?

Would you kindly elaborate?

Many thanks in advance,

 David L. Haviland, Ph.D.
 Asst. Prof. Immunology 
 University of Texas - Houston, H.S.C.
 Institute of Molecular Medicine  
 2121 W. Holcombe Blvd.  
 Houston, TX  77030 
 Internet:"dhavilan at" 
 Voice: 713.500.2413  FAX: 713.500.2424
" Sometimes you're the windsheild, sometimes you're the bug."

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