Shearing DNA

Jun-Zhi Wei jwei at sfu.ca
Wed May 7 18:56:52 EST 1997


> 
> Could you explain exactly how you did this? I'd like the same size
> fragments. (500bp). Thanks!
> 
> -- 
> Susan
> http://www4.ncsu.edu/unity/users/s/sjhogart/public/home.html
> 
> "To stay awake all night adds a day to your life."
>                        -- Stilgar (Frank Herbert)
> 

Susan:

Here is the method I used.

1. Dilute DNA to desired concentration;
2. Transfer the sample to 1.5 ml microtubes (no more than 0.5 ml in each
tube);
3. Close the cap tightly (use cap locker if necessary);
4. Autoclave on liquid cycle for 15 min at 15 lb/sq.in. 
5. Check with agarose gel.

Alternatively, you can put the tubes in boiling water for 45 min to obtain
around 500 bp fragments.

Hope this helps.

J.-Z. Wei



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