Shearing DNA

David L. Haviland, Ph.D. dhavilan at IMM2.IMM.UTH.TMC.EDU
Wed May 7 11:18:27 EST 1997


At 09:34 5/6/97 -0500, eric anderson wrote:
>In article <Pine.A41.3.95.970505172855.32796D-100000 at aix1.uottawa.ca>, ed
><s535290 at aix1.uottawa.ca> wrote:
>
>> Ian,
>> 
>> 
>> why don't you try nebulizing the DNA ?  someone in this department uses
>> nebulization very frequently and has his system finely tuned to generate a
>> median fragment size of about 3 Kb. He uses it for making shotgun
>> libraries for large scale sequencing. If you are interested, drop me a
>> line and I can pass this person's e-mail address to you. The procedure is
>> so foolproof that we used it on an undergraduate molecular biology lab :)
>> ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
>
>actually, foolproof is when it works in the hands of a rotation grad
>student _AND_ a postdoc.

Eric:

Umm... are you serious???  A nebulizer?   That noisy machine that
aerosolized albuterol for various members of my family when the pollens and
molds get too high?

Would you kindly elaborate?

Many thanks in advance,
David

=============================
 David L. Haviland, Ph.D.
 Asst. Prof. Immunology 
 University of Texas - Houston, H.S.C.
 Institute of Molecular Medicine  
 2121 W. Holcombe Blvd.  
 Houston, TX  77030 
 Internet:"dhavilan at imm2.imm.uth.tmc.edu" 
 Voice: 713.500.2413  FAX: 713.500.2424
 ------------------------------------------------------  
" Sometimes you're the windsheild, sometimes you're the bug."
=============================




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