crbaker at mail.tcd.ie
Thu May 8 10:59:06 EST 1997
Has anyone had experience with his-tagged proteins that precipitate
immediately on elution with imidazole? Is there a way to get around
Also are there any other tricks to removing backround contaminants
(under native conditions) apart from use of b-mercaptoethanol and
higher salt concs?
I guess that binding and elution parameters are protein specific but I
would appreciate any guidelines or tips.
Thanks in advance.
Dept. of Biochemistry,
Trinity College Dublin
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