His-tagged proteins

Cara Baker crbaker at mail.tcd.ie
Thu May 8 10:59:06 EST 1997


Has anyone had experience with his-tagged proteins that precipitate 
immediately on elution with imidazole? Is there a way to get around 
this?
Also are there any other tricks to removing backround contaminants 
(under native conditions) apart from use of b-mercaptoethanol and 
higher salt concs?
I guess that binding and elution parameters are protein specific but I 
would appreciate any guidelines or tips.

Thanks in advance.

Cara Baker,
Dept. of Biochemistry,
Trinity College Dublin



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