Buffer for lyophylization

Thorsten Schmidt Thorsten.Schmidt at rz.ruhr-uni-bochum.de
Fri May 9 12:59:41 EST 1997


Hi Mike!

> I am wondering if anyone knows which is the best buffer to lyophylize
from? 
> Excluding water, what buffer would leave beind the least amount of salt
> upon lyophylization?
> 

I´m not sure, but if you lyophylize I think only the water is removed.
I don´t know but how do you think salts are removed?

I would say that it must be preferable to use a buffer containing a low
salt concentration
to protect the proteins during lyophylization from degradation.

Thorsten



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