Ligating large fragments into pBluescript

Mark Leyland ml27 at le.ac.uk
Fri May 9 09:41:18 EST 1997


Hi

Has anybody got any experience of cloning quite large fragments of DNA
into plasmid vectors?
I'm trying to ligate fragments of 12kB and 18kb into pBluescript and
just wondered whether I was simply wasting my time or if it was possible
after all.
Any comments, tips or advice welcome.

Thanks in advance

Mark Leyland
Department of Cell Physiology and Pharmacology
University of Leicester
P.O. Box 138
Leicester
LE1 9HN

Tel 0116 2525586
FAX 0116 2525616
e-mail ml27 at le.ac.uk



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