Ligating large fragments into pBluescript
ml27 at le.ac.uk
Fri May 9 09:41:18 EST 1997
Has anybody got any experience of cloning quite large fragments of DNA
into plasmid vectors?
I'm trying to ligate fragments of 12kB and 18kb into pBluescript and
just wondered whether I was simply wasting my time or if it was possible
Any comments, tips or advice welcome.
Thanks in advance
Department of Cell Physiology and Pharmacology
University of Leicester
P.O. Box 138
Tel 0116 2525586
FAX 0116 2525616
e-mail ml27 at le.ac.uk
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