Someone stated recently that it is necessary to phosphorylate Pfu PCR products prior to e.g. EcoRV subcloning. Is this really necessary? And if so why? Thanks Dr. Fabian F. MOEBIUS Institut fuer Biochemische Pharmakologie Peter Mayr Str. 1 6020 Innsbruck AUSTRIA TEL ++43-512-507-3155 FAX ++43-512-588627