Phosphorylation of Pfu PCR-products

FABIAN MOEBIUS Fabian.Moebius at uibk.ac.at
Fri May 9 08:22:51 EST 1997


Someone stated recently that it is necessary to phosphorylate Pfu PCR 
products prior to e.g. EcoRV subcloning. Is this really necessary? 
And if so why?
Thanks
Dr. Fabian F. MOEBIUS
Institut fuer Biochemische Pharmakologie
Peter Mayr Str. 1
6020 Innsbruck
AUSTRIA
TEL ++43-512-507-3155
FAX ++43-512-588627



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