Phosphorylation of Pfu PCR-products

FABIAN MOEBIUS Fabian.Moebius at
Fri May 9 08:22:51 EST 1997

Someone stated recently that it is necessary to phosphorylate Pfu PCR 
products prior to e.g. EcoRV subcloning. Is this really necessary? 
And if so why?
Dr. Fabian F. MOEBIUS
Institut fuer Biochemische Pharmakologie
Peter Mayr Str. 1
6020 Innsbruck
TEL ++43-512-507-3155
FAX ++43-512-588627

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