His-tagged proteins

[Jared Head]

Cara Baker (crbaker at mail.tcd.ie) wrote:

: Has anyone had experience with his-tagged proteins that precipitate 
: immediately on elution with imidazole? Is there a way to get around 
: this?

I've had the same problem; my protein precipitates when I dialyse away the
imidazole.  I've found that if I elute the protein by stripping the column
(with EDTA) I get no precipitation at all.

Jared

-- 
Jared Head     at the Department of Biochemistry, University of Bristol

"A computer lets you make more mistakes faster than any invention in human
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