Clontech library problem
sltan at u.washington.edu
Fri May 9 14:27:35 EST 1997
i think clontech sells antibodies against the GAL4 BD and AD domains so
you can test expression of your hybrid proteins. I have also used
the yeast 2-hybrid system to pull out proteins that make a lot of sense
but they were all out of frame wrpt the vector sequence (pACT2). My guess
is that somehow the plasmid sequences were mutated when i passed
transferred them into E. coli (i used DH5a) for amplification and
On Fri, 2 May 1997, Jennifer Glick wrote:
> Hello netters!
> I have a Clontech two-hybrid (Matchmaker) library which is giving me
> fits. I have pulled out four clones which all make sense. However,
> three of the four are out of frame w/r/t the vector sequence from
> Clontech. While the clones are all proteins which have been identified,
> there are no antibodies for me to test if they are indeed what is being
> expressed. My question is, has anyone out there used this library???
> Its a human brain library in pACT2.
> Jennifer Glick jbelyea at acpub.duke.edu
> Duke University Medical Center (919) 613-8615 Tel.
> Durham NC 27707 (919) 613-8642 Fax.
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