HELP: Immunofluorescence

Martin Offterdinger a8803349 at unet.univie.ac.at
Mon May 12 04:16:34 EST 1997


Hi
I am just getting involved with immunofluorescence for the first time
and have some really basic questions:
1) Is there any good and recent review arcticle describing the whole
method.
2) Which fluorochrome should be chosen for parafin embedded
sections?(single labelling)
I learned that many people use fluorescein, but this fluorochrome
seems to be very sensitive to bleaching-so why is it still the most
commonly used?
I rather would like to use something else like Texas red or PE-is
there anyone who has experience with this??
Thank you very much, 
Martin



More information about the Methods mailing list