Degenerate Primer In PCR

Ma ddsyr at NS.EAST.CN.NET
Wed May 14 07:11:58 EST 1997

Dear Netters:

I have some questions about design the degenearate primers in PCR amplification:
(1) what is the maximas of degenerate rate (say in 20 bp oligo)?
(2) if the degenerate rate is greater, how to reduce it?
(3) in using the degenerate primers in PCR amplification, should we increase the amount 
of degenerate primers according its degenerate rate, so that to get the final products?
Anyone have any idea or experience on that? Thanks in advance.

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