Shearing DNA--Summary of responses

D. KIM dkim at
Tue May 13 17:40:57 EST 1997

WRT list of methods to shear DNA:

One method listed was nebulization.  I wonder if there is any hazard
associated with this method, since DNA delivered by inhalation of
liposomes in mice is sufficient to transfect the mouse's lung epithelial
cells with a marker gene.  This was a possible method for teatment/cure
for cystic fibrosis. (I forget the citation, sorry).

I don't want to be an alarmist, but I would avoid breathing large amounts
of the DNA mist while doing this procedure.

Daniel Kim
dkim at

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