fill-in and blunt-end ligation
oliver at molgen.biologie.uni-marburg.de
Wed May 14 03:16:14 EST 1997
Martin Offterdinger wrote:
> On 12 May 1997 22:29:18 GMT, Mari.L.Shinohara at dartmouth.edu (Mari L.
> Shinohara) wrote:
> >all different enzymes generating 5'-protruding ends. Then I
> >dephosphrylated only the vector fragment first, which has the Amp gene
> 1) It is very important to remove ALL alkaline phosphatase (normally
> calf intestine AP) before ligating, with two phenol extractions.
I have good experience with Shrimp alcaline phosphatase which can be
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