sjhogart at unity.ncsu.edu
Tue May 13 23:26:50 EST 1997
> On Tue, 13 May 1997 17:53:43 +0100, jmejia at worf.molbiol.ox.ac.uk (Jose
> E. Mejia) wrote:
> >I am looking for protocols or kits fo plasmid minipreps that do not
> >involve denaturation of DNA, i.e. neither alkaline nor boiling lysis. If
> >anyone knows about any such methods, please drop me a line.
> my favorite:
> 1. Grow cells overnight in LB at 37 C with shaking.
> 2. Microfuge cells for 30 sec. Remove supernatant by aspiration.
> 3. Resuspend cells in 100 uL TELT Buffer:
Sorry for the dumb question, but:
How/when is the plasmid DNA seperated from the genomic DNA in this prep?
"To stay awake all night adds a day to your life."
-- Stilgar (Frank Herbert)
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