PCR leaf

[Christian Meyer]

In article (Dans l'article) <3379E9A2.B5F at watstar.uwaterloo.ca>, Yvonne
Stevens <yysteven at watstar.uwaterloo.ca> wrote (écrivait) :

We used directly leaf pieces for genomic DNA amplification (Berthomieu and
Meyer, Plant Mol Biol 17:555-557, 1991). In our hands it works quite
well..
Good luck

Christian Meyer
Lab. Biologie Cellulaire
INRA Versailles FRANCE
meyer at versailles.inra.fr

> I have to screen many plants for presence of a gene. I am planning to do
> it by PCR (I know the sequence). What is the quickest way ? Is it
> possible to take small piece or peel from the leaf and add directly in
> the PCR mixture ? OR just grind the leaf, centrifuge or not, and add the
> paste/liquid in the PCR mixture ?
>   Please let me know your experience to my email:
> s3shah at sciborg.uwaterloo.ca
> 
> Thanks in advance.
> S. Shah

-- 
Christian MEYER
Email:meyer at versailles.inra.fr