Phosphorylation of Pfu PCR-products

Colin Rasmussen colin at pombe.biochem.ualberta.ca
Thu May 15 15:27:46 EST 1997


> 
> But be aware that your vector can also religate. If you have a good
> possibilty to select for clones with insert (blue/white), it's no big
> problem. Otherwise it can be a problem.

Or using a cloning kit like pCR-Script from Stratagene.


Colin
> 
> Hope that helps.
> 
> Oliver



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