plasma membrane prep

Seth Crosby crosby at
Thu May 15 12:57:45 EST 1997

I've been trying to find a simple way to prepare semi-pure plasma 
membranes from CHO cells.  These suckers are somewhat tough.  I find 
that a simple 1mM Tris solution with protease inhibitors coupled with a 
tight dounce works rather well.  30-40 strokes, 700G spin to pellet the 
nuclei, the a 11,000G spin to pellet the membranes.
Does any see anyposible problems with this protocol?  Got any better 
Thanks for your time!!

Seth Crosby, MD
Abbott Labs
Senior Research Scientist
Growth Factors Group

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