plasma membrane prep
crosby at milbrandt.wustl.edu
Thu May 15 12:57:45 EST 1997
I've been trying to find a simple way to prepare semi-pure plasma
membranes from CHO cells. These suckers are somewhat tough. I find
that a simple 1mM Tris solution with protease inhibitors coupled with a
tight dounce works rather well. 30-40 strokes, 700G spin to pellet the
nuclei, the a 11,000G spin to pellet the membranes.
Does any see anyposible problems with this protocol? Got any better
Thanks for your time!!
Seth Crosby, MD
Senior Research Scientist
Growth Factors Group
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